Journal: Scientific Reports
Article Title: Atorvastatin calcium alleviates UVB-induced HaCat cell senescence and skin photoaging
doi: 10.1038/s41598-024-81573-x
Figure Lengend Snippet: ( a ) Calcium atorvastatin’s chemical composition. ( b ) Plots stained with SA-β-Gal and varying amounts of Ato. ( c ) The proportion of cells positive for SA-β-Gal, as indicated in ( b ). ( d ) Viability of Hacat cells following the application of Ato (0–3 μM). ( e ) GAPDH and p16 Western Blot. ( f ) The quantification of p16 protein levels using pictures from three separate tests, as indicated in ( e ). ( g ) P21 mRNA level relative fold changes as assessed by qRT-PCR. In comparison to the vehicle control, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001.
Article Snippet: Anti-p16 (Cat#SR34-02, 1:1000) was purchased from Huabio (Hangzhou, China).
Techniques: Staining, Western Blot, Quantitative RT-PCR, Comparison, Control